RESUMO
In order to examine the genetic variation of clinical and food isolates of Listeria monocytogenes in Greece, a total of 61 L. monocytogenes non-duplicate isolates, recovered from clinical specimens (n=19) and food (n=42), were serotyped and genotyped using two different Random Amplification of Polymorphic DNA (RAPD) protocols and Multiple Locus Variable Number Tandem Repeat Analysis (MLVA). Serotype group 4b, 4d, 4e prevailed (39.4%), among both clinical and food isolates, followed by serotype group 1/2a, 3a (23.0%), which nevertheless was detected only among food isolates. The most discriminatory typing protocol was MLVA, which grouped four isolates into two pairs, while the remaining isolates produced unique fingerprints. Similar results were obtained when taking into account the combination of the two RAPD protocols (Simpson index 0.999); six isolates were grouped into three pairs, two of which were the pairs that were identified also by MLVA. Single use of each RAPD protocol resulted in inferior discrimination (Simpson index 0.978 and 0.997, respectively). In conclusion, the two molecular procedures, MLVA, and the combined RAPD protocols, produced similar results, showing that L. monocytogenes isolates from clinical and food specimens were highly heterogenous and that clustering was very uncommon.
Assuntos
DNA Bacteriano/genética , Microbiologia de Alimentos , Doenças Transmitidas por Alimentos/microbiologia , Variação Genética , Listeria monocytogenes/genética , Listeria monocytogenes/isolamento & purificação , Listeriose/microbiologia , Animais , Bacteriemia/sangue , Bacteriemia/líquido cefalorraquidiano , Bacteriemia/microbiologia , Análise por Conglomerados , DNA Bacteriano/metabolismo , Doenças Transmitidas por Alimentos/sangue , Doenças Transmitidas por Alimentos/líquido cefalorraquidiano , Grécia , Hospitais Urbanos , Humanos , Listeria monocytogenes/classificação , Listeria monocytogenes/metabolismo , Listeriose/sangue , Listeriose/líquido cefalorraquidiano , Carne/microbiologia , Tipagem Molecular , Reação em Cadeia da Polimerase Multiplex , Técnica de Amplificação ao Acaso de DNA Polimórfico , Sorotipagem , Sequências de Repetição em TandemRESUMO
Virgin olive oil is well known for its high content of phenolic substances that are thought to have health-promoting properties. These substances also contribute to the distinctive taste of the oil. In this study, tyrosol, vanillic acid, luteolin, and apigenin were identified and quantified by liquid chromatography mass spectrometry (LC-MS). In the seven samples analysed, tyrosol, the most abundant, was in the range of 1.4-29 mg/kg, vanillic acid was in the range of 0.67-4.0 mg/kg, luteolin was in the range of 0.22-7.0 mg/kg, and apigenin was in the range of 0.68-1.6 mg/kg. It was also shown that in olive oil, squalene can be analysed by using a refractive index detector. In the samples analysed, squalene occurred in the range of 3.9-9.6 g/l.
